Prolonged Transmission Of A Resistant Bacterial Strain

A strain is a low level taxonomic rank using at the intraspecific level; Strains are often seeing as inherently artificial concepts; characterizing by a specific intent for genetic isolation. This is most easily observing in microbiology where strains are deriving from a single cell colony; and are typically quarantining by the physical constraints of a Petri dish.

But these strains are also commonly referring to within virology, botany; and with rodents using in experimental studies. The gene armed the bacteria with resistance to carbapenems; a type of antimicrobial drug reserved as a last-line treatment for serious infections. Researchers have used whole genome sequencing (WGS); to demonstrate transmission of a single bacterial strain that possessed a carbapenem resistance gene.

Resistant bacterial strain

But epidemiologists from the local public health department; and the California Department of Public Health (CDPH) collaborated with laboratory scientists from the CDPH Microbial Diseases Laboratory (MDL); but to characterize the strain and identify potential transmission pathways in combination with epidemiologic information.

But bacterial strain persisted in the hospital for over 3 years; potentially due to transmission from patient to patient combining with lengthy stays in the hospital intensive care unit (ICU). Study investigation highlights the importance of regular epidemiological; and microbiological monitoring of resistant strains in hospitals; and the use of the advanced molecular technologies to track their spread; Between 2013 and 2015, the hospital identified eight patients; who were positive for a strain of Klebsiella pneumoniae bacteria that produced an enzyme conferring resistance to carbapenems.

Monitoring of resistant strains

But the initial four patients identifying with the resistant strain had overlapping staying in the ICU over a one-month period. One of these initial patients remained in the ICU for two years; during which time an additional four patients with the resistant bacterial strain were finding in the facility. WGS analyses of the bacteria from the eight different patients demonstrating they were all highly related genetically.

WGS also showed that this particular bacterial strain’s carbapenem resistance gene; appearing to be locating on the chromosome. Normally, such resistance genes are located on additional “mobile” DNA pieces called plasmids; that can be transferring between different strains (and even different species) of bacteria. Using WGS to track resistant bacterial strains can help hospitals; and public health officials target infection control interventions to halt transmission sooner.