Imagine a day when a bioprinter filled with a patient’s own cells can be wheeled right to the bedside; to treat large wounds or burns by printing skin, layer by layer, to begin the healing process. That day is not far off. Wake Forest Institute for Regenerative Medicine (WFIRM) scientists; have created such a mobile skin bioprinting system—the first of its kind—that allows bi-layered skin to be printed directly into a wound.
“The unique aspect of this technology is the mobility of the system and the ability to provide on-site management of extensive wounds; by scanning and measuring them in order to deposit the cells directly where they are needed to create skin,” said Sean Murphy, Ph.D., a WFIRM assistant professor who was lead author of the paper published this month in Nature’s Scientific Reports journal.
Multiple treatments for wound healing
Affecting millions of Americans, chronic, large or non-healing wounds such as diabetic pressure ulcers are especially costly; because they often require multiple treatments. It is also estimated that burn injuries account for 10-30% of combat casualties in conventional warfare for military personnel.
The major skin cells; dermal fibroblasts and epidermal keratinocytes; are easily isolated from a small biopsy of uninjured tissue and expanded. So, fibroblasts are cells that synthesize the extracellular matrix and collagen that play a critical role; but in wound healing while keratinocytes are the predominant cells found in the epidermis; the outermost layer of the skin.
The cells are mixing into a hydrogel and placing into the bioprinter. But integrated imaging technology involving a device that scans the wound; hence feeds the data into the software to tell the print heads which cells to deliver exactly where in the wound layer by layer. Doing so replicates and accelerates the formation of normal skin structure and function.
Scaning the wound
But the researchers demonstrated proof-of-concept of the system by printing skin directly onto pre-clinical models. The next step is to conduct a clinical trial in humans. Hence, skin grafts to treat wounds and burns are the “gold standard” technique; but adequate coverage of wounds is often a challenging; particularly when there is limiting availability of healthy skin to harvest.
Skin grafts from donors are an option, so risk immune rejection of the graft and scar formation. With the WFIRM bioprinter system the researchers could see new skin forming outward; from the center of the wound happening when the patient’s own cells were using; because the tissues were accepting and not rejected.
Painful skin grafts
“The technology has the potential to eliminate the need for painful skin grafts; but that cause further disfigurement for patients suffering from large wounds or burns,” said WFIRM Director Anthony Atala, M.D.; and a co-author of the paper. “A mobile bioprinter that can provide on-site management of extensive wounds; could help to accelerate the delivery of care and decrease costs for patients.”
“If you deliver the patient’s own cells, they do actively contribute to wound healing; but by organizing up front to start the healing process much faster,” said James Yoo, Ph. D, who led the research team and co-authored the paper. “While there are other types of wound healing products available to treat wounds; and help them close, those products do not actually contribute directly to the creation of skin.”