The researchers are in the process of developing innovative solutions that will ensure CRISPR-Cas9 can be safely used in a range of clinical applications. Their report on the potential benefits and risks of CRISPR-Cas9 can be found in the current issue of Nature Medicine
CRISPR-Cas9 is a new molecular gene editing technique that allows scientists to make specific changes to the DNA of humans, other animals, and plants. The technique is analogous to a pair of molecular scissors that enable scientists to cut, silence or replace specific DNA sequences.
The CRISPR-Cas9 gene editing technology can be used in vitro or in vivo-ie, it can be used to modify cells outside the body, or it can be delivered to the body to modify specific target cells. Both applications have demonstrated the effectiveness and ease of use in animal models.
However, there is insufficient data to allow researchers to assess the risks and benefits of using this technology in humans. By studying immune responses to the CRISPR- Cas9 system , a research team, led by Dr. Michael Schmück-Henneresse of Charité's Berlin-Brandenburg Center for Regenerative Therapies (BCRT), has succeeded in closing this knowledge gap.
"The Cas9 protein, which is derived from Streptococcus bacteria, forms an integral part of the CRISPR-Cas9 system. As streptococcal infections are common in humans, we hypothesized that there would be a pre-existing immunological memory to Cas9," explains Dr. Schmück-Henneresse. T cells (human immune cells) that react to Cas proteins were found in almost all of the healthy human subjects tested.
Cas molecules derived from other bacteria, such as staphylococci or gastrointestinal bacteria, also generate this type of immune reaction-a phenomenon that may be due to a high degree of similarity between these enzymes.
"These types of immune cells can produce unwelcome immune responses during gene therapy, thus potentially impairing the technology's safety and efficacy." CRISPR-Cas9 May also harbor this risk, and it is something we need to prepare for, "says the study's first author , Dimitrios Laurin Wagner, a doctoral student at the BCRT.
This means that cells that have been modified in vitro should not be injected into patients in whom CRISPR-Cas9 remains active. "We have developed a test that will allow us to ensure that we can use that device to reliably determine if the risk of an immune reaction is low," explains Dr. Schmück-Henneresse.
However, some genetic diseases produce tissue defects that can not be modified outside the human body. New solutions must be found to prevent negative immune responses to the CRISPR-Cas9 gene editing scissors.
Working with colleagues from the Berlin Center for Advanced Therapies (BeCAT), the researchers have developed a technology that will ensure the safety of CRISPR-Cas9-based therapies. This technology works by isolating and expanding regulatory T cells , which control unwanted immune responses. Initial clinical testing of this technology, used in patients who had undergone either kidney or bone marrow transplants, has produced positive results.