Hematology

The study find that the body for mechanical stimuli such as touch, bladder fullness, and blood pressure. The discovery is a feat of basic science that also opens up many new paths of investigation into the roles of Piezo proteins in human diseases and potential new therapeutic strategies. But In the study, published Aug. 21 in Nature; the scientists used advanced microscopy techniques to image the Piezo1 protein at rest and during the application of mechanical forces. Therefore They confirmed this complex protein’s structure and showed essentially how it can convert mechanical stimuli into an electrical signal.

Blood vessel-lining cells

“Our analysis shows that tension on the cell membrane in which Piezo1 is embedded can flatten and widen the protein’s structure;” said co-senior author Dr. Simon Scheuring; a professor of physiology and biophysics in anesthesiology at Weill Cornell Medicine. Dr. Scheuring and his laboratory collaborated on the study with the laboratory of Dr. Roderick MacKinnon; a professor of molecular neurobiology and biophysics at The Rockefeller University. Dr. MacKinnon was co-recipient of the Nobel Prize in Chemistry in 2003 for his work determining the structures and mechanisms of ion channel proteins.

Piezo1 and Piezo2 are very large and complex proteins with unique structures. They are embedded within the membranes of certain cell types, and their function is to transduce mechanical force on cells into electrical signals that alter cell activity. Piezo1 proteins work for example in bladder cells to detect when the bladder is full, and in blood vessel-lining cells to detect and help regulate changes in blood pressure. Because Piezo2 proteins work in sensory nerve endings in the skin and joints, helping to mediate the senses of touch, pain, and proprioception  the sense of how one’s limbs are arranged.

Transduce mechanical force

Advances in imaging techniques have enabled scientists in recent years to determine the basic structure of Piezo1 a structure that Piezo2 is thought to mostly share. But From above this structure has a three-armed, propeller or “triskelion” appearance. From the side it looks like a shallow bowl embedded in the cell membrane; with an ion channel at its center. The latter, when opened, allows a flow of calcium and other positively charged ions into the cell.

The basic mechanism by which mechanical force opens the ion channel has remained mysterious. But in the new study Dr. Scheuring and Dr. MacKinnon and their colleagues, including lead author Dr. Yi-Chih Lin, a postdoctoral associate in anesthesiology, were able to get a clearer picture of how it works.