Human cells shed exosomes. These tiny extracellular, membrane-bound vesicles can carry cargo for cell-to-cell communication, ferrying diverse loads of proteins, lipids or nucleic acids. University of Alabama at Birmingham and Chinese researchers now report that exosomes are key to the SOS signal that the heart muscle sends out after a heart attack.
After the heart attack, the exosomes in the bloodstream carry greatly; hence increased amounts of heart specific microRNAs an observation seen in both mice and humans. These exosomes preferentially carry the microRNAs to progenitor cells in the bone marrow. Inside those progenitor cells, the microRNAs turn off a specific gene that allows the progenitor cells to leave the bone marrow and enter the bloodstream.
The cells then travel to the heart to attempt repairs. The investigators say discovery of this novel pathway a signal from the damage heart to a systemic response by the reparative bone marrow cells can now be leverage to improve cell-based cardiovascular repair after heart attacks.
For 15 years, it had been known that progenitor cells are release from the bone marrow after a heart attack. These cells move to the damaged heart muscle to attempt repairs. However, many efforts to improve that repair have yielded only modest efficacies, at best. Similarly,for dozen years, it was known that a handful of microRNAs are abundant in heart muscle are greatly elevate in the bloodstream after an acute heart attack; that both in animals and in humans.
The UAB and Chinese researchers have now linked those two observations by identifying the cargo carrier that ferries those heart muscle microRNAs, hence locating the preferred destination of the cargo carrier and describing the microRNA mechanism that releases the progenitor cells from the bone marrow.
The researchers found that, after heart attack in a mouse model; so levels of four heart-muscle-specific microRNAs called myo-miRs were elevated 10,000 to 100,000-fold in peripheral blood after heart attack. These myo-miRs was preferentially took up by bone marrow mononuclear cells, and to a lesser extent, kidney cells.
MicroRNAs, or miRNAs, are small, non-coding RNAs that can regulate gene expression. The human genome appears to make at least 2,000 different miRNAs. It turned out that exosomes in the blood overwhelmingly carried three of the myo-miRs after a heart attack miR 1a, miR-208a and miR-499-5p. The fourth myo-miR, miR-133a, was partially carrying by exosomes. Notably, a significant increase of circulating exosome myo-miRs was also seen in humans; so after the serious heart attacks called acute ST-elevation myocardial infarction.
Transformation of exosomes
Exosomes from non-heart-attack mice did not carry the myo-miRs. The researchers found that both the myo-miR exosomes, from after heart attacks; the non-heart-attack exosomes readily transferred into bone marrow mononuclear cells. It was known as blockade of a chemokine receptor called CXCR4; that mediates mobilization of bone marrow progenitor cells into the blood circulation and contributes to repair of heart muscle after heart attacks.
Since one of the myo-miRs showed a potential binding to the CXCR4 mRNA; all four of the myo-miRs was testing on bone marrow mononuclear cells. Surprisingly, each myo-miR alone could downregulate expression of CXCR4. Furthermore, exosomes from heart attack mice but not from non-heart-attack mice downregulated CXCR4 in bone marrow mononuclear cells.
Finally, researchers showed that exosomes from heart attack mice; when transferred to intact mice, significantly reduced CXCR4 expression in bone marrow mononuclear cells and caused mobilization of progenitor cells out of the bone marrow. Thus, overall, the injured heart is signalling to mobilize the bone marrow progenitor cells.
There work reveals that exosomes released from the ischemic heart are in fact mediating the systemic response of bone marrow progenitor cells to the site of injury; the researchers reported. These observations are compelling, as circulating myo-miRs are not only a bio-marker; but also a functional constituent of the intricate crosstalk between the ischemic heart and the remote bone marrow organ.
Because of the critical role of bone marrow progenitor cells in the ischemic tissue repair; they said, the mechanism identified in this study could be an important component of the physiological response to ischemic injury and thus could be leveraged for treatment of ischemic heart disease.