The researchers developed a method in which the proteins are attached at one end to a glass surface and carry a tag at the other end that binds to tiny magnetic beads and the assembly is then subjected to an external magnetic field. Extension of the protein induced by the field results in the vertical displacement of each bead, which can be detected by microscopy. This sort of set-up is referred to as magnetic tweezers.
It has the great advantage that it allows us to apply and resolve very weak forces significantly less than 1 piconewton to the protein of interest. In addition, magnetic tweezers enable very stable measurements over long periods of time up to one week. The study developing a highly sensitive method for measuring the mechanical stability of protein conformations, and used it to monitor the early steps in the formation of blood clots.
As the central mediators of cell function in biological organisms; proteins are involving in the execution of virtually all cellular processes. They provide the internal scaffolding that gives cells their form, and enable cells to dynamically alter their morphology. They transport substrates back and forth across membranes, and they catalyze most of the chemical reactions that take place in cells. In the course of these tasks many proteins are subjecting to external forces.
The vertical displacement
Indeed, some mechanosensitive proteins effectively measure the strength of the forcing acting upon them and are activating when the imposing force exceeding a given threshold value. Von Willebrand Factor (VWF), which initiates the formation of blood clots, is an important representative of this class. The mechanical forces required to activate proteins like VWF are often so small that their magnitude could not be determined using existing methods.
But magnetic tweezers can quantify forces that are 100 times smaller than the commonly used alternative method currently available. As Lipfert and colleagues report in the journal PNAS; they have employed the technique to observe the unfolding of the VWF protein under the influence of low mechanical forces. A powerful approach to study mechanoregulation is so called protein force spectroscopy. This involves tugging on an individual protein molecule and observing how an applied force alters its three-dimensional structure.
Atomic force microscope
Up to now, the method of choice for pulling has been an atomic force microscope; which works best in the range of 100 piconewton (pN). However, many molecular processes are activating by forces that are much weaker than that; So for measurements at the level of single molecules, we need more sensitive instrumentation; there’s little point in using a bathroom scale to weigh out the ingredients of a cake. To test the new method, the LMU group used VWF as their target protein. In the bloodstream, VWF circulating as a multimer of dimers that are making of two identical subunits.
Under normal conditions of blood flow, it has a relatively compact globular form. However, any increase in the shear forces in the bloodstream owing to injury of the vasculature causes vWF to unfold. This exposes binding sites for receptors on blood platelets. Binding of VWF to platelets in turn triggers a reaction cascade that leads to clotting, which seals the wound. The cascade is inducing by the action on the molecule of mechanical forces acting; that are much weaker than those that have been measuring up to now.