At the 2018 American Society of Cell Biology Meeting (ASCB), Bruker today announced the launch of the new  Luxendo InVi SPIM AIM  next-generation lattice light-sheet microscope which features an advanced illumination module (AIM) for lowest phototoxicity light-sheet fluorescence microscopy of live samples.

Leveraging the general benefits of single-plane illumination microscopy (SPIM) with high-photon efficiency and short illumination times, the new module enables the user to interactively customize the light-sheet shape to the system's tailor to particular specimen's requirements. A variety of illumination patterns, including single or multiple variable Bessel beams, lattice light sheets, and structured illumination, provide a much greater range of single-instrument research possibilities for rapid, higher resolution 3D imaging of living cells.

The  Luxendo InVi SPIM AIM  uses the high-performance platform for gentle long-term imaging with precise control of physiological conditions. It has been designed to combine the advantages of several different illumination approaches to deliver the required flexibility to further optimize bioimaging experiments, from a large field of view and extraordinarily high temporal sampling to spatial resolution at the physical limit.

"The new Advanced Illumination Module dramatically expands the capabilities of our SPIM systems with a choice for the optimal light-sheet for the experimental requirements, leading to better solutions," said Dr. Andreas Pfuhl, Vice President and General Manager of Bruker's Luxendo business "This next-generation, lattice light-sheet microscope gives scientists, for the first time, the freedom to customize the light sheet and tune the microscope to a specific biological application, providing advantages in live-cell investigations with higher resolution." 

About the Luxendo InVi SPIM AIM

The Luxendo InVi SPIM AIM is a next-generation, lattice light-sheet fluorescence microscope based on the InVi platform. It provides all the necessary capabilities to improve spatial resolution and further reduce photodamage in live-cell imaging of delicate samples to resolve subcellular structures and dynamics.

The system maintains the ease of use and stability of the InVi SPIM while enabling illumination of the sample with flexible light-sheet patterns. These patterns include the classical static Gaussian light-sheet and the scanned Gaussian beam, as well as sophisticated illumination schemes like Bessel beams or lattice light-sheets, improving the microscope's resolution in time and space while minimizing phototoxic effects.

The light-sheet geometry can be adapted to the sample in a secure, utterly computer-controlled manner to offer cutting-edge, high-resolution imaging in an unprecedented user-friendly framework.

About Luxendo

Headquartered in Heidelberg, Germany, Luxendo was founded in September 2015 as a spin-off of the European Molecular Biology Laboratory (EMBL). Luxendo was able to rapidly develop robust product solutions based on the patented SPIM technology. Now, as part of Bruker Corporation, Luxendo's light-sheet microscopes join Bruker's existing portfolio of swept-field confocal, super-resolution, and multiphoton fluorescence microscope product lines, enabling new research advances in small organism embryology, live-cell imaging, brain development and cleared brain tissue, and optogenetics applications. For more information