Where fenpropathrin (FNP) is a member of the synthetic pyrethroids; Here in, the present study conducting to investigate; for the first time, the potentially harmful effects of FNP on the reproductive system testicular damage of male rats; A wide range of synthetic pesticiding have been leaking into the rural environment through the agricultural activities to control plant pathogens; insect pests and weeds not only in the developing countries but in the developing ones as well.
Humans and animals are continually exposed to traces of PYRs via food and water of plant origin; Fenpropathrin (FNP) is a member of the synthetic pyrethroids; FNP modify the gating kinetics of the voltage sensitive sodium channels; thereby they interrupt nerve function producing acute neurotoxic outcomes in both insects and non target organisms; There is a growing concern about the PYRs abuse in veterinary medicine which in turn leads to subacute and chronic effects in nontarget organisms.
Immunohisto chemical staining for Caspase 3 proteins and P53 were doing according to ABC technique describing; Briefly, paraffin embedding tissue sections of 3μ thickness; were deparaffinizing in xylene and then rehydrated in ethyl alcohol. The sections were incubating for 2 h with 5% bovine serum albumin in Tris-buffering saline (TBS) for blocking of the non-specific binding sites.
Throughout the experiment, animals from the control; FNP and/or milk exposing groups were ostensibly normal except the increasing level of salvation in the FNP exposing group. No mortalities during the period of the experiment were recording. Presenting data showing that FNP administration significantly decreasing the final body weight; testis weight and the gonadosomatic index of the treating rats when comparing to other groups.
Inhibition of spermatogenesis
The main factor affecting testes weight is the inhibition of spermatogenesis, steroidogenic enzyme activity and reduction of the number of germ cells which is obvious in the results of this study as a consequence of FNP; intoxication. Other pyrethroids such as cypermethrin and deltamethrin have been reporting to generate oxidative stress in cells. Oxidative damage may be a key mechanism of FNP -induced apoptosis.
These effects may be through the DNA damaging effect of FNP and mitochondrial induction pathway of apoptosis in spermatogonial cells of rats. Conversely, CM could effectively arrest the FNP induced damage. The prophylaxis routine of CM administration was obviously effective than co-treatment manner.