Novel Therapeutic Approaches For Life Threatening Inflammatory

By novel therapeutic approaches for better understanding the life-threatening inflammatory diseases such as sepsis, pointing to a biochemical agent that may be involved in the rapid decline in blood pressure that occurs in the advanced stage of sepsis and usually causes the patient’s death.

A metabolic product of the amino acid tryptophan was believing to be one of the factors that cause vasodilation ;(widening of blood vessels) and sharply falling blood pressure during sepsis;Under inflammatory conditions; this haem-containing enzyme is expressing in arterial endothelial cells; where it contributes to the regulation of blood pressureThis study showing  that the problem causing by singlet oxygen; an electronically excited state of oxygen.

Therapeutic Approaches

Singlet oxygen is a highly reactive molecule with two pairing electrons; in the same orbital or different orbitals.Chemically generated  O2 oxidizes the amino acid tryptophan; to precursors of a key metabolite called N-formylkynurenine4 ;whereas enzymatic oxidation of tryptophan to N-formylkynurenine is catalysing by a family of dioxygenases; including indoleamine 2,3-dioxygenase .

Singlet oxygen is involved in the formation of a signaling biomolecule that regulates vascular tone and blood pressure during the inflammation characteristic of sepsis.the levels of H2O2 in blood vessel walls rise very sharply during life-threatening inflammatory processes such as sepsis, reaching extremely high levels. In addition, tryptophan is pumped to these arterial endothelial cells.

Liquid Chromatography

The research steps involving experiments like nuclear magnetic resonance and liquid chromatography coupled with mass spectrometer; the researchers demonstrated the formation of a different vasodilator from the one previously identified. The compound; which they call cis-WOOH, is forming by the enzyme indoleamine 2,3-dioxygenase in a reaction involving tryptophan an essential amino acid; found in proteins and singlet oxygen in the presence of high levels of hydrogen peroxide.

But the biochemical component responsible for the fall in blood pressure in sepsis and on new biological functions for the enzyme IDO1; could serve as a starting point for the discovery of a refined repertoire of redox signaling paths in which reactive oxygen species such as singlet oxygen act as biological messengers.