Researchers found a rare genetic disorder in which people are suddenly overcome with profound muscle weakness is caused by a hole in a membrane protein that allows sodium ions to leak across cell membranes. A hole in a protein lets ions leaks into cells, thereby triggering the profound muscle weakness of hypokalemic periodic paralysis. The study was published in the journal Nature.

The condition is called hypokalemic periodic paralysis. People who have this disorder also occasionally have low blood levels of potassium, a state called hypokalemia. The disease was known to stem from mutations that alter certain cell membrane proteins, but how the mutation changes the proteins' function was not known. Our results reveal the mechanisms of periodic paralysis at the atomic level and suggest designs for drugs that may prevent this ion leak and provide relief to these patients.

This difference is maintained by a system of ion pumps that move ions in and out of the cell. Of these, the sodium-potassium pumps, which move sodium out of the cell in exchange for potassium, are especially important. The interior of the cell is more negatively charged than the exterior because potassium continuously flows out of the cell. This charge difference generates a voltage across the membrane of about 90 millivolts in skeletal muscle.

In this process, chemical signals from a nerve cause chemically sensitive ion channels to open. Sodium ions then flow into the cell and lower the voltage difference across the nearby membrane. This change in voltage, in turn, is sensed by nearby voltage-gated sodium channels.

If the voltage change is large enough, sodium channels are triggered to open. More sodium flows into the cell. This process creates a change in the membrane potential that ripples down the length of the muscle cell. The "action potential" that is generated triggers muscle contraction. The mutation that causes hypokalemic periodic paralysis affects a key functional part of the voltage-gated sodium channel, called the voltage sensor.

It detects and responds to changes in membrane voltage by changing its molecular shape. The reconfiguration causes the channel to open and permits sodium ions to pass into the cell. In their study, the researchers used a technique called X-ray crystallography to determine the structure of the normal and mutated versions of the voltage sensor at atomic resolution. They found that mutations responsible for hypokalemic periodic paralysis create a small hole through the center of the voltage sensor in the sodium channel protein.

Under ordinary circumstances, the ion channels that allow potassium to flow out of cells and the sodium-potassium ion pumps can make up for this leakage. The researchers also showed that compounds containing a chemical group called guanidinium could block the pore created by the mutation and stop the sodium leak without altering the voltage sensor's ability to function.

Our high-resolution structural models may provide templates for drugs that mimic the effect of guanidinium, block the gating pore current, and could perhaps prevent or treat periodic paralysis.