Jellyfish respond quickly to external stress that stimulates mucus secretion as a defense. Neither the composition of secreted mucus nor the process of secretion is well understood.
Aurelia coerulea jellyfish were stimulated by removing them from environmental seawater. Secreted mucus and tissue samples were then collected within 60 min, and analyzed by a combination of proteomics and metabolomics using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) and ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS), respectively.
In this study, we have explored the stress-induced mucus secretion and its constituent composition in the jellyfish A. coerulea by a combination of proteomics and metabolomics.
The first conclusion is that two different but successive phases have been drawn from the initial stress to the final autolysis and death, with a distinct inflection point occurring at 30 min after removing the jellyfish from seawater.
The results of proteomics using GO and KEGG analyses drew our second conclusion that the proteins in stress-induced mucus are independent (i.e., different) to those in tissue homogenate.
It was also identified that the mucus-enriched proteins are mainly located in the extracellular or membrane-associated region, while the tissue-enriched proteins are located in the intracellular compartment.
The results of metabolomics are of particular interest; the potential neurotransmitter or neuromodulator, tryptamine, displays the maximal FC value of 7.8, a significantly elevated value among 16 other different metabolites in stress-induced mucus compared to those of tissue homogenate. This supports the hypothesis that a drastic nerve stress response, as well as a tempestuous release of neurotransmitters, occurs upon stress initiation.
Finally, 11 metalloproteinases, four serine proteinase inhibitors, three SODs, three compliments, and four toxin-related proteins were successfully assigned to function as self-protective components. In summary, our results provide a constituent profile of proteins and metabolites in stress-induced mucus and tissue homogenate of A. coerulea.
This profile is, we believe, particularly important in equipping us with a better understanding of the process of stress-induced mucus secretion, as well as signaling the important role these self-protective components play in the innate immunity of jellyfish and the ongoing discovery of novel bioactive compounds. This study provides insight for the ongoing endeavors to discover novel bioactive compounds.
The large increase of tryptamine in mucus may indicate a strong stress response when jellyfish were taken out of seawater and the active self-protective components such as enzymes, serpins and complements potentially play a key role in innate immunity of jellyfish.