Researchers used a computational method to redesign aspartase and convert it to a catalyst for asymmetric hydroamination reactions. Their colleagues in China scaled up the production of this enzyme and managed to produce kilograms of very pure building blocks for pharmaceuticals and other bioactive compounds. They used a computational method to redesign aspartase and convert it to a catalyst for asymmetric hydroamination reactions. The study was published in Nature Chemical Biology.
Enzymes are natural catalysts that work under mild conditions. They are an attractive alternative to uncatalyzed chemical reactions that often require energy-consuming high temperatures or pressure and may generate toxic side products or use solvents. But there is one problem: the range of reactions catalyzed by enzymes is limited. 'That's why a lot of effort is being put into modifying natural enzymes.'
The classic method to modify enzymes is directed evolution, a mutation-selection sequence in the lab which aims to create enzymes with modified catalytic abilities. But it takes a lot of work to make and test hundreds or thousands of enzyme variants in multiple rounds. It would be much more efficient to make a rational design of the required changes that are based on information on the enzyme's structure and properties.
But even this is complicated, explains Hein Wijma. He is an expert in molecular design software and did most of the computational work in the study. 'Proteins are made of 20 different amino acids. So if you want to change an enzyme in four positions, there are 20 options for each of them. That results in a huge matrix of protein structures.' Testing them one by one, even on the computer, takes too long. However, they search algorithm speeds up the discovery of the right outcome by looking for trends in the enzyme's reactivity.
They have to model the reactive center, the pockets where the substrate binds, and determine the distance between the amino acids and their relative positions and angles.' As the group used one enzyme as a starting point for a number of different reactions, the starting point was always the same. This meant they only had to change the target reaction. 'If they wanted to make a new modification of aspartase, that would probably just take three months now.'
The research paper describes four different conversions, all additions of ammonium. Aspartase is a deaminase, so the reaction was reversed. 'Catalysis goes both ways, so that is not a major problem', the initial selection by the algorithm produced some 100 promising mutants. These were then checked for obvious errors.
The next step was to test successful mutant enzymes in a scaled-up setting. 'This work was done by a former Ph.D. student and postdoc from our lab, Bian Wu, who is now an assistant professor in China'. They showed which candidates could produce large quantities of the required product.'
The author concludes that substrate conversions of 99% with a 99% enantioselectivity were achieved in quantities up to a kilogram, meaning that the enzymes predicted by the computation methods appear suitable for use in an industrial setting. 'This is real proof of principle that our method of in-silico selection of mutants works for producing useful enzymes.