In the paper published in journal Nature Communications, researchers demonstrated the design and synthesis of a smart globular macromolecular machine vehicle for actively controlled cancer drug delivery, which would enhance the drug's efficacy. This breakthrough gives insight to targeted therapy drugs such as Chlorambucil in the treatment of leukaemia.
The team reported on a series of novel hyperbranched macromolecules with at most 15 mechanical bonds at the branching unit: mechanical bonds are a novel and exciting class of non-covalent bonds similar to familiar chains and hooks, for example. These macromolecules can induce an overall extension-contraction molecular motion via collective and controllable molecular back-and-forth shuttling, providing the ability to encapsulate drug molecules and release them actively by acidic stimuli.
In current leukaemia treatment, drugs are delivered to kill leukaemia cells that may be present in the blood and bone marrow. However, the number of drugs released to kill the free-floating cancer cells cannot be effectively controlled. The 15 mechanical bonds resemble 15 mechanical arms that actively control the delivery and suitable amount of drugs released to targeted cancer cells. This smart material combines molecular machines and dendrimers with a new breakthrough in synthesis as well as controlled and active drug release.
With its complexity and size, this synthetic molecule resembles a small virus. Due to the relatively low toxicity of this smart globular molecular vehicle, it can also serve as a potential ideal long-term drug delivery molecular machine submerged in the human body. The molecular masses of these new macromolecules were characterised by mass spectrometry, and their chemical structures and physical properties were also verified with supercomputer simulations.
The syntheses of macromolecular machines are complicated whereas the intermediate compounds require the formation of mechanical bonds followed by careful purification and characterisation. Molecular machines are assembled with their molecular counterparts that are responsive to specific stimuli (input) and produce mechanical movements (output). Rotaxane dendrimers are molecular interlocked molecules that combine hyperbranched macromolecules with molecular machines.
Among various types of rotaxane dendrimer, type III-B possesses the most complicated molecular structure and exhibits the largest extension-contraction properties. The breakthrough in the synthesis and the control of particle size of higher-generation rotaxane dendrimers could give scientists an insight to develop more sophisticated molecular machines to be applied in functional materials and nanotechnology, such as the delivery of drugs or biomolecules.