In a new study published in the Marine Drugs, researchers have reported that preliminary studies in vitro on fibroblast cell cultures demonstrated the good compatibility of platelet lysate with nanocarriers and bioactive dressings. An in vivo study on a murine wound model showed an accelerating wound healing effect for the bioactive dressing and its suitability as support of the platelet lysate application to wounds.

Chitosan is endowed with wound healing promotion effect, due to its ability to enhance growth factor and cytokine expression and to promote the stability of growth factors. Among the more recent therapeutic strategies for chronic wounds, there is the topical employment of platelet derivatives, whose therapeutic role in tissue regeneration, due to the release of growth factors (GFs) and cytokines, is widely recognized.

Some studies highlight moreover the relationship between platelet GFs and the control of oxidative stress in wound healing. The aim of the present work was the development of a bioactive dressing containing silver sulfadiazine (AgSD) as anti-infective drug and alpha-tocopherol (αTph) as an antioxidant agent and intended to be loaded with autologous PL in the treatment of chronic skin wounds.

As both AgSD and αTph are poorly soluble, chitosan oleate was previously proposed to encapsulate in nanocarriers some poorly soluble molecules aimed to wound therapy, such as the anti-infective silver sulfadiazine, and the antioxidant α tocopherol. Because nanocarriers need a suitable formulation to be administered to wounds, in the present paper, these previously developed nanocarriers were loaded into freeze-dried dressings based on chitosan glutamate.

These were proposed as bioactive dressings aimed to support the application to wounds of platelet lysate, a hemoderivative rich in growth factors. The dressings were characterized for hydration capacity, morphological aspect, and rheological and mechanical behavior. Although chitosan oleate nanocarriers clearly decreased the mechanical properties of dressings, these remained compatible with handling and application to wounds.

The study results show that the encapsulation of poorly soluble hydrophobic molecules in chitosan oleate allows easy dispersion in hydrophilic formulations such as freeze-dried dressings. The combined application of platelet lysate (PL) with bioactive 2 mg dressing (PL-BD) significantly improves the dressing effect and an improvement of PL efficacy on wound healing promotion are also suggested. The bioactive dressing can, therefore, be a suitable and versatile support of the hemoderivative for the treatment of wounds. The dose-dependent effect of the bioactive dressing makes it a flexible tool in wound treatment. Moreover, as PL preparation involves freezing steps, hemoderivative doses can be safely stored, handled and associated with the dressings without efforts by caregivers or patients.