New research has uncovered the mechanism used by cells to optimize the production of proteins in stressful situations by altering tRNA abundance. The regulating mechanism may play a vital role in preventing the proliferation of tumors.
Researchers from the Universitat Autònoma de Barcelona, and the Medical Research Council Cambridge (UK), for the first time, describe the mechanism used by cells to optimize the production of proteins in stressful situations by altering tRNA abundance.
The different genes within the genome must be expressed in precise levels and the exact moment if the complex balance regulating cell activity is to be maintained. The messenger RNA (mRNA) conveys genetic information from DNA to ribosomes, where proteins are synthesized through the union of amino acids.
These amino acids are supplied by fragments of transfer RNA (tRNA), which decode the information contained within the mRNA codons (a group of three bases sequencing each amino acid) to chain them correctly and synthesize each protein.
Production Of Proteins
The study headed by researchers Marc Torrent from the Department of Biochemistry and Molecular Biology of the Universitat Autònoma de Barcelona, and Madan Babu from the Medical Research Council Cambridge, United Kingdom, for the first time describes the mechanism used by cells to optimize the production of proteins in stressful situations by altering tRNA abundance.
The research, conducted with Saccharomyces cerevisiae yeast, suggests that the mRNAs coded by proteins needed in times of stress prefer a different set of codons. This is correlated to the changes produced in the relative abundance of the different tRNAs.
These observations suggest that the cell is capable of regulating the abundance of different tRNAs and adjusting the level of protein expression while adapting to the new conditions. Thus, the change in abundance in different tRNAs is an independent mechanism of regulation allowing a selective modification of translation rates and, therefore, can increase or diminish the relative abundance of the proteins needed in different conditions of stress.
These observations have very notable implications. On the one hand, the regulation mechanism is important because the alteration of tRNA abundance can cause diverse cell dysfunctions. Other studies already observed that the overexpression of the transfer RNA iMet and Brf1, a transcription factor of the complex RNA polymerase III, causes the formation of tumors in vivo.
On the other hand, the tRNA is also related to apoptosis, programmed cell death which halts the proliferation of tumors. It has been demonstrated that the tRNA can join the cytochrome complex and prevent the activation of caspases, protease enzymes playing essential roles in programmed cell death.
Therefore, the levels of tRNA in the cell also can play a relevant role in the ability of cells to respond to apoptotic stimuli related to the proliferation of tumors.