A new study from the University of Pennsylvania found that early childhood caries result from dental plaque that contains both bacteria and fungus working together to make the biofilm on the teeth more pathogenic and difficult to remove. Now they have shown that these two types of microorganisms synergize to enhance drug resistance, enabling the fungal cells to avoid being killed by antifungal therapies. Yet simultaneously targeting the matrix produced by the bacteria along with the fungus offers a way around this protection.
During the last several years, researchers have observed that the dental plaque in children with early childhood caries often contained Candida albicans, a fungal species that normally colonizes mucosal surfaces, in addition to Streptococcus mutans, the bacteria generally associated with tooth decay.
New study demonstrated that an enzyme produced by the bacteria, termed GtfB, can bind to Candida and when sugar is present a sticky polymeric matrix forms on its cell surface, enabling the fungus to bind to teeth and associate with bacterial counterparts. Once together, these organisms work in concert to increase severity of tooth decay in a rodent model.
Realizing this,research geam led by Hyun (Michel) Koo, a professor in the Department of Orthodontics and divisions of Pediatric Dentistry & Community Oral Health in Penn's School of Dental Medicine, wanted to see whether a two-pronged approach might break apart the synergistic association and effectively treat the biofilm. "Initially, we decided to look into therapies that are clinically used in dentistry to attack or prevent either fungal or bacterial infections," Koo says.
They came up with fluconazole, which is used as an antifungal, and povidone iodide, which is an antiseptic agent with antibacterial properties. Used alone to treat biofilms grown on a tooth-like material in the lab, the drugs had only moderate effects, confirming that monotherapy doesn't work very well against polymicrobial biofilms. But in combination, the results were much more impressive.
The researchers looked closely at high-resolution microscopic images of the biofilms with the various treatment combinations. They observed that, in untreated biofilms and those treated with solely fluconazole, the fungus was coated with abundant sticky matrix, which seemed to serve as a protective shield against the antifungal compound. But in biofilms treated with povidone iodide as well, the matrix was substantially reduced, leaving the fungus exposed to the fluconazole.
"We thought, that's interesting," Koo says, and turned to the scientific literature to find out more. They discovered that iodide-containing drugs can inhibit the activity of GtfB. In a series of experiments, they found that povidone iodide acted as a powerful inhibitor of the sticky-matrix production. The agent was almost 100-fold more potent as an inhibitor of the matrix than it was as an antibacterial agent.
That led them to the hypothesis that the matrix was serving as a "drug-trapping shield," preventing the fluconazole from accessing and killing the fungal cells. To see whether disrupting the matrix could allow the fluconazole to penetrate and reach the fungus, they collaborated with Tel Aviv University scientists to track, in real time, fluorescently-labeled fluconazole as it moved through a biofilm.
Taking time-lapsed images, they found that the fluconazole were trapped in the matrix, largely failing to reach the fungal cells, which was further confirmed by directly measuring radiolabeled fluconazole absorbed in the matrix. In contrast, fluconazole readily moved inside the fungal cells when they were located in biofilms with the matrix disrupted by povidone iodine.
Using three different assays to disrupt the matrix, either by directly degrading the matrix or using bacteria defective in GtfB, the researchers found that the antifungal-killing ability of fluconazole could be completely restored, confirming the role of the bacteria-produced matrix in promoting antifungal drug resistance.
Looking ahead, the Penn-led team hopes their findings lead to new strategies for treating bacterial-fungal infections associated with early childhood caries and possibly other polymicrobial diseases. For the researchers' part, they are making use of nanotechnology to develop targeted approaches that can precisely target the matrix and both the fungal and bacterial components of the oral biofilm.?