New research shows the most common Parkinson's gene mutation may change how immune cells react to generic infections like colds, which in turn trigger the inflammatory reaction in the brain that causes Parkinson's disease
"We know that brain cells called microglia cause the inflammation that ultimately destroys the area of the brain responsible for movement in Parkinson's," said Richard Smeyne, Ph.D., Director of the Jefferson Comprehensive Parkinson's Disease and Movement Disorder Center. "But it wasn't clear how a commonly inherited mutation was involved in that process, and whether the mutation altered microglia."
Elena Kozina, Ph.D. et al. looked at the mutant version of the LRRK2 gene. Mutations in the LRRK2 gene are the most common cause of inherited Parkinson's disease.
LRRK2 genes are found in 40 percent of people of North African Arab descent and 18 percent of people of Ashkenazi Jewish descent with Parkinson's. However, there has been controversy around the exact function of the LRRK2 gene in the brain.
"We know that gene mutation is not enough to cause the disease," said Dr. Kozina, a Post-Doctoral student at Jefferson. "We know that twins who both carry the mutation, won't both necessarily develop Parkinson's. A second 'hit' or initiating event is needed."
Based on the researcher's previous work showing that the flu might increase the risk of Parkinson's disease, Dr. Smeyne decided to investigate whether that second hit came from an infection.
Suspecting that the LRRK2 mutations might be acting outside of the brain, the researchers used an agent — the outer shell of bacteria, called lipopolysaccharide (LPS) – that causes an immune reaction. LPS itself does not pass into the brain, nor do the immune cells it activates, which made it ideal for testing whether this second hit was acting directly in the brain.
When the researchers gave the bacterial fragments to the mice carrying the two most common LRRK2 gene mutations, the immune reaction became a "cytokine storm," with inflammatory mediators rising to levels that 3-5 times higher than a typical response to LPS. These inflammatory mediators were produced by T and B immune cells expressing the LRRK2 mutation.
Despite the fact that LPS did not cross the blood-brain barrier, the researchers showed that the elevated cytokines were able to enter the brain, creating an environment that caused the microglia to activate pathologically and destroy the brain region involved in the movement.
"Although more tests are needed to prove the link, as well as testing whether the same is true in humans, these findings give us a new way to think about how these mutations could cause Parkinson's," said Dr. Smeyne. "Even though we can't treat people with immunosuppressants their whole lives to prevent the disease if this mechanism is confirmed, it's possible that other interventions could be effective at reducing the chance of developing the disease," he added.